Plasmid

Part:BBa_K2563001

Designed by: Charles Thomas Anderson   Group: iGEM18_iTesla-SoundBio   (2018-10-14)


pAX01 w/xylR+PxylA

This would be a BioBrick-compatible version of the Bacillus integration vector pAX01. Unlike BBa_J179001--to which it is otherwise identical--it would include the xylose-inducible xylR+PxylA repressor-promoter system specified by the part BBa_K733002. This would allow transcriptional regulation of the cloned gene through the addition and removal of xylose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 4560
    Illegal NotI site found at 6091
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 6646
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3593
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1441
    Illegal SapI.rc site found at 2523


[edit]
Categories
Parameters
None